Equipment Sterilization Protocols

Why Sterile Technique Matters

Peptide solutions are excellent growth media for bacteria. A single contamination event can:

• Render your peptide unusable
• Produce harmful endotoxins
• Consume peptide as a nutrient source
• Produce enzymes that degrade the peptide

Proper sterilization and aseptic technique prevent these issues and protect your investment.

Workspace Preparation

Surface Cleaning

• Wipe down work surface with 70% isopropyl alcohol
• Allow to air dry completely (at least 1 minute)
• Use lint-free wipes to avoid leaving fibers
• Repeat before each session

Creating a Clean Field

• Lay out a clean, disposable paper towel or sterile drape
• Organize all supplies before beginning
• Keep contaminated items separate from clean supplies
• Work away from air vents or drafts

Vial and Stopper Sterilization

Pre-Sealed Vials (Recommended)

Pre-sealed sterile vials with rubber stoppers are the safest option. Simply wipe the stopper with an alcohol swab before puncturing.

Empty Vials for Custom Solutions

• Purchase pre-sterilized vials whenever possible
• If sterilizing yourself, autoclave glass vials at 121°C for 15 minutes
• Rubber stoppers can be autoclaved separately or purchased sterile
• Allow to cool completely before use

Personal Protective Equipment

Gloves

• Use powder-free nitrile or latex gloves
• Change gloves if they touch non-sterile surfaces
• Spray gloves with 70% alcohol before handling sterile materials

Additional PPE for Sensitive Work

• Lab coat or clean long sleeves
• Face mask (reduces contamination from breathing)
• Hair covering if working near open containers

Syringe and Needle Handling

• Always use individually wrapped, sterile syringes and needles
• Never reuse needles between vials
• Do not touch the needle with anything except the vial stopper
• If the needle touches any surface, replace it immediately
• Dispose of sharps in proper sharps containers