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CJC-1295 (No DAC)

Technical Monograph

CJC-1295 (without DAC, strictly modified GRF 1-29) is a synthetic analogue of endogenous Growth Hormone Releasing Hormone (GHRH). By incorporating specific amino acid substitutions at positions 2, 8, 15, and 27, the peptide achieves significant resistance to dipeptidyl peptidase-IV (DPP-IV) cleavage.

Mechanism of Action

The peptide functions as a potent agonist of the GHRH receptor in the anterior pituitary. Upon structural binding, it stimulates the adenylyl cyclase/cAMP and phospholipase C pathways simultaneously. This results in the transcription of the GH1 gene and the direct release of growth hormone.

Pharmacological Detail

Selective activation of GHRH-receptors utilizing the required 29-amino-acid active core.

Pharmacodynamics

Restores or accelerates somatotropic axis function. Promotes transcription-level synthesis of GH.

Pharmacokinetics

Without strict DAC conjugation, half-life is restricted to roughly 30 minutes. Represents a significant improvement over natural GHRH (7 minutes).

Preclinical Observations & In Vitro Data

Extensively studied for somatopause reversal and tissue anabolism.

Preclinical (Swine): Sustained elevation of pulsatile GH secretion with peak amplitudes elevated 3-fold.

Observed Timeline of Action

TimeframePharmacological Effect
15-30 minsPeak plasma action; somatotrope receptor saturation.
1-2 hoursGH spike resolution; return to elevated basal output.

Abstract Highlights

  • Resists rapid degradation by serum DPP-IV via D-Ala substitution at position 2
  • Increases basal and pulsatile GH secretion symmetrically
  • Synergistically amplifies total GH output when administered with a GHRP

References

Teichkoetter T, et al. (2006)."Prolonged stimulation of growth hormone and IGF-1 secretion by CJC-1295."J. Clin. Endocrinol. Metab.

Chemical Specifications

3367.9 g/mol
C152H252N44O42
Tyr-D-Ala-Asp-Ala-Ile-Phe-Thr-Gln-Ser-Tyr-Arg-Lys-Val-Leu-Ala-Gln-Leu-Ser-Ala-Arg-Lys-Leu-Leu-Gln-Asp-Ile-Leu-Ser-Arg-NH2

Material Handling & Stability

Laboratory StorageLyophilized powder: Stored securely at -20°C.
Aqueous StabilityReconstituted: highly stable at 2-8°C. Estimated working viable lifespan of 14-21 days.

Strict Notice

THIS COMPOUND IS PRODUCED FOR LABORATORY RESEARCH PURPOSES ONLY. NOT FOR HUMAN CONSUMPTION. MATERIAL DATA SHEETS AND LABORATORY GUIDELINES SHOULD BE CONSULTED PRIOR TO HANDLING.